Journal: Cell Transplantation

Article Title: HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways

doi: 10.1177/0963689719883578

Figure Lengend Snippet: Expression of Wnt3a, BMP2 and β-catenin nuclear proteins in NSCs after HBO treatment by Western blotting. (A) Expression of Wnt3a and BMP2 protein: HBO treatment up-regulated the expression of Wnt3a and BMP2 proteins. (B) Expression of β-catenin nuclear and cytoplasmic proteins: HBO up-regulated the expression of β-catenin nuclear protein; however, the expression of β-catenin cytoplasmic protein was not affected. (C and D) Each bar represents mean±SD, n = 3. a p < 0.05 vs. the CON group, ANOVA test; b p < 0.05 vs. the HBO group, ANOVA test; c p < 0.05 vs. the NBH group, ANOVA test; d p < 0.05 vs. the HIBH group, ANOVA test. NSCs: neural stem cells; HBO: hyperbaric oxygen; CON: control; NBH: normal brain tissue extracts; HIBH: hypoxic-ischemic brain damage tissue extracts; BMP2: bone morphogenetic protein.

Article Snippet: The following primary antibodies were used: Rabbit mAb active Wnt3 (1:100; SC-28824, Santa Cruz, Biotechnology, Inc., Dallas, TX, USA), rabbit mAb active β-catenin (1:100; BA0426, Wuhan Boster Biological Technology, Ltd.), rabbit mAb active BMP2 (1:100; PB0727, Wuhan Boster Biological Technology, Ltd.), mouse mAb β-actin (1:200, BM0626, Wuhan Boster Biological Technology, Ltd.) and mouse mAb active PCNA (1:1000; 610664, BD Biosciences, San Jose, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cell Transplantation

Article Title: HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways

doi: 10.1177/0963689719883578

Figure Lengend Snippet: Expression, after HBO treatment, of Wnt3a, BMP2 and β-catenin nuclear proteins in the presence of sFRP2/3 was determined by Western blotting. (A) Expression of Wnt3a and BMP2 proteins: sFRP2/3 reduced the expression of Wnt3a and BMP2 proteins after HBO treatment. (B) Expression of β-catenin nuclear and cytoplasmic proteins: sFRP2/3 reduced the expression of β-catenin nuclear protein after HBO treatment; the expression of β-catenin cytoplasmic protein was not affected. (C and D) Each bar represents mean±SD, n = 3. a p < 0.05 vs. the HIBD+HBO group, ANOVA test; b p < 0.05 vs. the HBO group, ANOVA test. HBO: hyperbaric oxygen; CON: control; HIBD: hypoxic-ischemic brain damage; sFRP2/3: secreted Frizzled-related protein 2 and 3; BMP2: bone morphogenetic protein.

Article Snippet: The following primary antibodies were used: Rabbit mAb active Wnt3 (1:100; SC-28824, Santa Cruz, Biotechnology, Inc., Dallas, TX, USA), rabbit mAb active β-catenin (1:100; BA0426, Wuhan Boster Biological Technology, Ltd.), rabbit mAb active BMP2 (1:100; PB0727, Wuhan Boster Biological Technology, Ltd.), mouse mAb β-actin (1:200, BM0626, Wuhan Boster Biological Technology, Ltd.) and mouse mAb active PCNA (1:1000; 610664, BD Biosciences, San Jose, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Determination of β-catenin Expression in Breast Cancer and Its Relationship with Clinicopathologic Parameters

doi: 10.31557/APJCP.2021.22.11.3493

Figure Lengend Snippet: The Frequency of Different β-catenin Expression Patterns in Breast Cancer Specimens

Article Snippet: IHC was performed using Rabbit anti-human β-catenin monoclonal antibody ( clone EP35, Master diagnostica) according to the manufacturer’s protocol.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Determination of β-catenin Expression in Breast Cancer and Its Relationship with Clinicopathologic Parameters

doi: 10.31557/APJCP.2021.22.11.3493

Figure Lengend Snippet: Patterns of β-catenin Expression and Its Relationship with Age and Sex of the Patient

Article Snippet: IHC was performed using Rabbit anti-human β-catenin monoclonal antibody ( clone EP35, Master diagnostica) according to the manufacturer’s protocol.

Techniques: Expressing

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: Silencing of DYNLL1 reduces malignant phenotype of LUAD cells and blocks cell cycle progression. Three KD-DYNLL1 plasmids (KD-DYNLL1 1#, 2#, 3#) were transfected into NCI-H1395 and NCI-H441 cells using lentiviral vectors. A DYNLL1 mRNA levels in transfected cells were measured by qPCR, with KD-DYNLL1 2# exhibiting the strongest knockdown effect, selected for subsequent experiments. B Colony formation assay to evaluate the colony-forming ability of KD-DYNLL1-transfected cells. C Immunofluorescence staining for Ki67 to assess cell proliferation potential. D Flow cytometry analysis of cell apoptosis. E Wound healing assays to evaluate cell migration. F Transwell assay to assess cell invasion. G Flow cytometry analysis of cell cycle distribution. H Western blot analysis of protein expression levels of Cyclin D1, p21, and β-catenin in transfected cells. Each dot corresponds to one independent experiment. Differences were analyzed by two-way ANOVA ( A – H ) * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: Transfection, Knockdown, Colony Assay, Immunofluorescence, Staining, Flow Cytometry, Migration, Transwell Assay, Western Blot, Expressing

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: DYNLL1 overexpression activates the Wnt/β-catenin pathway and rescues malignant properties of LUAD cells suppressed by TEAD4 knockdown. NCI-1395 and NCI-H441 cells transfected with KD-TEAD4 were additionally administered OE-DYNLL1. A DYNLL1 mRNA expression in cells measured by qPCR analysis. B Colony formation assays to evaluate the colony-forming ability of treated cells. C Immunofluorescence staining for Ki67 to assess cell proliferation. D Flow cytometry analysis of cell apoptosis. E Flow cytometry analysis of cell cycle distribution. F SA-β-Gal (SABG) staining to analyze cell senescence. G Western blot (WB) analysis of Cyclin D1 and p21 protein levels. Each dot corresponds to one independent experiment. Differences were analyzed by two-way ANOVA ( A – G ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: Over Expression, Knockdown, Transfection, Expressing, Immunofluorescence, Staining, Flow Cytometry, Western Blot

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: TEAD4 and DYNLL1 affects tumorigenesis of LA795 cells in mice. Mouse LUAD cells (LA795; 5 × 10 6 cells per mouse) stably transfected with KD-TEAD4 and OE-DYNLL1 were implanted into BALB/c mice to induce allograft tumors. A Tumor volume measured over a 5-week period. B Representative images of tumors and tumor weight after 5 weeks of growth. C Western blot (WB) analysis for the expression of β-catenin, TEAD4, and DYNLL1 in tumor tissues. D Immunohistochemistry (IHC) for the positive staining of proliferation markers Ki67 and PCNA in tumor tissues. E IHC for the positive staining of Cyclin D1 and p21 levels in tumor tissues. F TUNEL assays to detect cell apoptosis within the tumor tissues. Differences were analyzed by two-way ANOVA ( A – F ). Each group contained six mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: Stable Transfection, Transfection, Western Blot, Expressing, Immunohistochemistry, Staining, TUNEL Assay

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: Graphic Abstract. TEAD4 regulates DYNLL1 transcription to activate the Wnt/β-catenin signaling, thus promoting cell cycle progression and the malignant properties of LUAD cells

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Tanshinone IIA promotes the proliferation of WB-F344 hepatic oval cells via Wnt/β-catenin signaling

doi: 10.3892/mmr.2015.4696

Figure Lengend Snippet: TSA increases the protein expression levels of β-catenin in WB-F344 oval cells. The WB-F344 oval cells were treated with 0 (control), 10, 20 and 40 µ g/ml TSA and analyzed following 24–96 h. Total proteins were extracted for (A) immunoblotting of β-catenin and β-actin. (B) Relative protein levels of β-catenin were normalized to β-actin. Data are expressed as the mean standard deviation. * P<0.05 and ** P<0.01, vs. control. TSA, tanshinone IIA.

Article Snippet: The primary antibodies used were as follows: Rabbit polyclonal anti-rat β-catenin (1:400; cat. no. BS3603; Bioworld Technology, Inc., St. Louis Park, MN, USA) and mouse monoclonal anti-rat β-actin (1:2,000; cat. no. A5441; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Tanshinone IIA promotes the proliferation of WB-F344 hepatic oval cells via Wnt/β-catenin signaling

doi: 10.3892/mmr.2015.4696

Figure Lengend Snippet: TSA upregulates the protein expression levels of β-catenin in WB-F344 oval cells. (A) WB-F344 oval cells were treated with 0 (control), 10, 20 and 40 µ g/ml TSA and analyzed after 24–96 h. Immunofluorescence was used to assess the expression β-catenin (red). Cell nuclei were counter-stained blue with DAPI. Magnification, ×600; scale bar, 50 µ m. (B) Number of β-catenin-positive stained cells in five randomly-selected fields were counted and plotted. Data are expressed as the mean standard deviation. * P<0.05 and ** P<0.01, vs. control. TSA, tanshinone IIA.

Article Snippet: The primary antibodies used were as follows: Rabbit polyclonal anti-rat β-catenin (1:400; cat. no. BS3603; Bioworld Technology, Inc., St. Louis Park, MN, USA) and mouse monoclonal anti-rat β-actin (1:2,000; cat. no. A5441; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Expressing, Immunofluorescence, Staining, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Tanshinone IIA promotes the proliferation of WB-F344 hepatic oval cells via Wnt/β-catenin signaling

doi: 10.3892/mmr.2015.4696

Figure Lengend Snippet: TSA upregulates the mRNA expression of β-catenin in hepatic cells. The WB-F344 oval cells were treated with 0 (control), 10, 20 and 40 µ g/ml TSA and analyzed following 24–96 h. Total RNAs were extracted for reverse transcription-quantitative polymerase chain reaction. β-catenin served as an internal control. Data are expressed as the mean standard deviation. * P<0.05 and ** P<0.01, vs. control. TSA, tanshinone IIA; mRNA, messenger RNA.

Article Snippet: The primary antibodies used were as follows: Rabbit polyclonal anti-rat β-catenin (1:400; cat. no. BS3603; Bioworld Technology, Inc., St. Louis Park, MN, USA) and mouse monoclonal anti-rat β-actin (1:2,000; cat. no. A5441; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Endocrine Connections

Article Title: The emerging role of the molecular marker p27 in the differential diagnosis of adrenocortical tumors

doi: 10.1530/EC-13-0025

Figure Lengend Snippet: β-Catenin staining localization distribution in the different study groups.

Article Snippet: The sections were incubated overnight at 4 °C with the appropriate diluted primary antibodies: rabbit anti-human MABs to p53 (453M-94; 1:100; Cell Marque, Rocklin, CA, USA), p21 (421M-14; 1:50; Cell Marque), p27 (427M-94; 1:500; Cell Marque), cyclin D1 (271R-14; 1:500; Cell Marque), Ki-67 (27R-14; 1:100; Cell Marque), and E-cadherin (246R-14; 1:200; Cell Marque) and rabbit anti-human polyclonal antibodies to β-catenin (424A-14; 1:500; Cell Marque).

Techniques: Staining

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Spatially restricted dental regeneration drives pufferfish beak development

doi: 10.1073/pnas.1702909114

Figure Lengend Snippet: Localization of a dental progenitor cell niche within the pufferfish dental lamina. (A–C) P. baileyi PCNA immunohistochemistry reveals high levels of cellular proliferation within the oral epithelium. As replacement teeth progress from late-initiation (A) to morphogenesis (C), the new tooth generation (R1) buds from the dental lamina (B). Successive rounds of replacement show that the dental generations stack on one another within an enameloid outer casing (black line) (C). (D) Sox2 immunohistochemical labeling during dental replacement initiation depicts high levels of Sox2 within both the developing taste buds (TB) and the dental progenitor site located within the labial oral epithelium (dental lamina) (black arrowhead). (E) Double immunofluorescence treatment for Sox2/PCNA in T. niphobles shows low levels of PCNA expression within the Sox2+ cells of the presumptive dental progenitor niche and cells within the aboral dental lamina exhibiting high levels of PCNA. The horizontal dashed line depicts image stitching of two adjacent images. White arrowhead marks region of overlapping PCNA/Sox2 expression. (F) BrdU pulse/chase experiments (0.2 mM) show the incorporation of BrdU into dividing cells after 6 wk of treatment, with high levels of incorporation noted in the distal dental lamina next to the base of the beak (white arrowhead). (G) After a further 8-wk chase, label-retaining cells were found in the most superficial dental lamina cells (open arrowhead) but not in the distal dental lamina (white arrowhead). Label-retaining cells found in the dental epithelium of the developing tooth are indicated by a white arrow. Images in F and G are composites of multiple images taken at high magnification and stitched together. (H) DiI labeling of the labial oral epithelium in P. suvattii highlighted this region as a presumptive source of dental progenitor cells. DiI was detected within the outer dental epithelium of the tooth (white arrowhead) 72 h post DiI treatment. (I) As summarized in a schematic representation, we observed a continuous field of Sox2+ cells between the labial taste bud and the dental progenitor site, with cells from the latter migrating and contributing to the new dental generations. Black arrows represent the direction of cell movement. (J) Sox2/ABC double immunohistochemical labeling on adult C. travancoricus highlights epithelial Sox2+/ABC− (a′, white filled arrow), Sox2+/ABC+ (b′, white arrow), and Sox2−/ABC+ (c′, white arrowhead) regions within the dental lamina. Coexpression of these markers marks the site of activation of putative dental progenitors within the oral epithelium. Dashed line across (J) depicts image stitching of two adjacent images. Images are orientated with labial to the left and oral to the top. The dotted line in all images depicts the boundary of the oral epithelium and the end of the dental lamina. DM, dental mesenchyme; ODE, outer dental epithelium; R1–3, replacement tooth generations; RT, regenerating tooth; TB, labial taste bud. (Scale bars: 25 µm in A–E; 20 µm in F, a′–F, c′; 50 µm in G and H; 15 µm in I.)

Article Snippet: Rabbit anti-Sox2/mouse anti-PCNA and rabbit anti-Sox2/mouse anti-active β-catenin (1:500) (05-665; Merck) double immunofluorescence was carried out as described by Martin et al. ( 17 ).

Techniques: Immunohistochemistry, Immunohistochemical staining, Labeling, Immunofluorescence, Expressing, Pulse Chase, Activation Assay

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Spatially restricted dental regeneration drives pufferfish beak development

doi: 10.1073/pnas.1702909114

Figure Lengend Snippet: Conserved odontogenic signaling regulates dental regeneration in pufferfish. (A–I) Expression of well-documented odontogenic markers belonging to Sox (sox2, A); canonical Wnt signaling (β-catenin, B; lef1 C); Pitx (pitx2, D); Shh (E), Notch (hes1, F; notch3 G); Bmp (bmp2, H); and Fgf (fgf3, I) gene families in T. niphobles embryos. The thin arrow marks the site of presumptive dental progenitors, with expression of pitx2 (D), lef1 (C), and sox2 (A) within this region. The thick arrow marks the distal end of the dental lamina. The filled arrowhead highlights an opening within the osteodentine beak casing through which new odontogenic cells bud from the dental lamina. β-cat (B), shh (E), hes1 (F), notch3 (G), bmp2 (H), and fgf3 (I) are all expressed within the epithelium of the latest developing teeth. (J) A diagrammatic illustration of odontogenetically similar structures in various polyphyodonts [pufferfish, alligator (7, 16), cichlid (5), and catshark (43)]. Four main developmental regions are highlighted: presumptive dental progenitors, progenitor cell activation marked by the coexpression of Sox and Wnt signals, dental epithelium differentiation marked by the up-regulation of various developmental genes at the distal tip of the dental lamina and the growth of a tooth bud, and dental morphogenesis. The dotted line depicts the boundary of the oral epithelium and the end of the dental lamina. All images were taken from 14-µm sagittal paraffin-embedded sections. A, B, E, F, H, and I are from T. niphobles embryos at 50 dpf. C, D, and G are from embryos at 32 dpf. R1-2, replacement tooth generations; S, suture; TB, labial taste bud. (Scale bars: 50 µm in B–D, F, and G; 35 µm in A, E, H, and I.)

Article Snippet: Rabbit anti-Sox2/mouse anti-PCNA and rabbit anti-Sox2/mouse anti-active β-catenin (1:500) (05-665; Merck) double immunofluorescence was carried out as described by Martin et al. ( 17 ).

Techniques: Expressing, Activation Assay